Effects of mTOR Transfection on Proliferation of NIH3T3 Fibroblasts
- VernacularTitle:mTOR基因转染对NIH3T3成纤维细胞增生的影响
- Author:
Xiaoming LI
;
Zuocheng YANG
;
Chunyuan CHEN
;
Zhuoying LI
- Publication Type:Journal Article
- Keywords:
mTOR;
Gene transfection;
Fibroblasts;
Cell proliferation
- From:
Journal of Medical Research
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of mammalian target of rapamycin(mTOR)transfection on the proliferation of NIH3T3 fibroblasts.Methods The plasmid of pcDNA3-mTOR was transfected into NIH3T3 fibroblasts with electroporation method.The positive cell clones were selected with G418.The stable mRNA and protein expressions of mTOR in the cells were determined by RT-PCR and Western blotting analysis respectively.MTT assay was employed to observe the proliferation of NIH3T3 fibroblasts.Results mTOR mRNA and protein expressions increased obviously in transfected group than that of in control group(P
