Optimization of Experimental Conditon in the PEG Precipitation Associated with Electrochemiluminescence Test to Detect Macroprolactinaemia
- VernacularTitle:聚乙二醇沉淀联合电化学发光法检测巨泌乳素血症实验条件优化
- Author:
Jiefeng DING
;
Rongfu ZHANG
- Publication Type:Journal Article
- Keywords:
Pre-treatment method;
Macroprolactinemia;
Optimization of conditon
- From:
Journal of Medical Research
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The experimental condition of PEG precipitation pre-treatment method associated with electrochemiluminescence was optimized to gain a coincident result compared with gel chromatography method.Methods A fixed centrifuge time,optimized centrifuge speed,temperature and iron intensity were used to achieve a best experimental condition.Results The detection concentration of micromolecular PRL was approximate to gel chromatography method under the experimental condition of being centrifuged 10 minutes at 18℃ and pretreated with 25%PEG 6000 1M PBS.Conclusions The optimized PEG precipitation test united with the electrochemillumescence immunoassy was a good method for screening macroprolactinemia in clinical diagnosis.
