The Time-dependent Manner of Reversible Effect of DDPH on Post-proliferation of PASMC
- VernacularTitle:DDPH逆转增生后PASMC的时间相关性研究
- Author:
Bing DU
;
Beibei CHEN
;
Ximing WANG
;
Qiuhong DUAN
;
Shanshu HE
- Publication Type:Journal Article
- Keywords:
DDPH;
?-smooth muscle-actin;
Pulmonary arterial smooth muscle cell;
Phenotype transformation;
Hypoxia
- From:
Journal of Medical Research
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of hypoxial endothelia cell conditional medium(HECCM)on the proliferation of pulmonary arterial muscle cell(PASMC).Methods MTT assay was used to test the proliferation,immuno-cytochemistry was used to identify the expression of ?-SM-actin.Results(1)HECCM could promote the proliferation of PASMC,down-regulate their expression of ?-SM-actin.(2)DDPH could up-regulate the expression of ?-SM-actin in PASMC which was time-dependant.Conclusions DDPH could reverse the phenotype transformation of PASMC exposed to HECCM,the action was time-dependant.After some time DDPH could reversly transform PASMC to the normal contractile phenotype.
