Changes in protease in ischemia-reperfusion lung injury and the protective effect of protease inhibitor in rabbit
- VernacularTitle:兔缺血再灌注肺损伤中蛋白酶变化及蛋白酶抑制剂的保护作用
- Author:
Tingting CHEN
;
Gang WANG
;
Changqing GAO
- Publication Type:Journal Article
- Keywords:
reperfusion injury;
acute lung injury;
peroxidase;
leukocyte elastase;
cytokines
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the changes in protease and cytokines,and to evaluate the protective effect of ulinastatin on ischemia-reperfusion lung injury in rabbits.Methods Thirty New Zealand rabbits were randomly divided into three groups(n=10): control group,traumatic hemorrhagic shock group(shock group) and ulinastatin-treated group.Blood samples were taken at four time points: pre-shock,post-shock,as well as 2h and 4h after blood volume repleshment.The serum levels of TNF-? and IL-8 were measured.4h after blood volume repleshment,the rabbits were sacrificed.The activity of myeloperoxidase(MPO) in lung tissue and neutrophil elastase(NE) in bronchoalveolar lavage fluid(BALF) were determined.Results In control group,the serum concentrations of TNF-? and IL-8 showed no obvious changes during the experiment.While in shock group and ulinastatin-treated group,the serum concentrations of TNF-? and IL-8 increased significantly in the duration of experiment compared with the values before shock(P
