Expression and methylation of ARHI gene in ovarian serous carcinoma
- VernacularTitle:印迹抑癌基因ARHI在浆液性卵巢癌中的表达及甲基化研究
- Author:
Wenjie SHEN
;
Fuqi XING
;
Yongxian LU
- Publication Type:Journal Article
- Keywords:
imprinted gene;
genes,ARHI;
ovarian neoplasms;
DNA methylation
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of ARHI mRNA,an imprinted suppressor gene,in ovarian serous carcinoma and the methylation status of its three CpG islands,and to determine the possible role of aberrant methylation of ARHI gene in pathogenesis of ovarian serous carcinoma.Methods Twenty normal ovarian specimens and 20 ovarian serous carcinoma specimens were analyzed.Total RNA was extracted and semi-quantitative RT-PCR was employed to detect the mRNA expression of ARHI gene.Methylation status of three CpG islands were examined by DNA bisulfite treatment,PCR amplification and methylation specific restriction enzymes,TagI for CpG I and III,BstUI for CpG II.Results The average of ARHI/G3PDH was 0.73?0.09 in normal ovarian specimens,and it was 0.43?0.37 in ovarian carcinoma specimens(P
