Effect of WenyangHuoxueFang on expression of MMP-3 and TIMP-1 in kidney of rats with unilateral ureteral obstruction(UUO)
- VernacularTitle:温阳活血方对单侧输尿管梗阻模型大鼠肾组织基质金属蛋白酶抑制剂-1和基质金属蛋白酶-3表达的影响
- Author:
Wei CAO
;
Jun LI
;
Jingli SHI
- Publication Type:Journal Article
- Keywords:
WenyangHuoxueFang;
Renal tubulointerstitial fibrosis;
MMP-3;
TIMP-1
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the protective effect of WenyangHuoxueFang on renal interstitial fibrosis in rats and try to explore the mechanism.Methods: The renal obstruction model of rats was established by unilateral ureteral obstruction(UUO) ,and then they were divided into four groups randomly,including UUOW group(treated with WenyangHuoxueFang),UUOF group(treated with Fosinopril),UUO group and the sham-operation group.The morphological changes of kidney tissue were observed,the level of RBP was detected by ELISA,the expression of MMP-3 and TIMP-1 was detected by immuno-histochemistry.Results: Compared with the sham-operation group,there were different degrees of kidney lesions in UUO group and the treatment groups.The level of RBP in UUO group was significantly higher than that in sham-operation group.Compared with UUO group,the RBP expression in UUOF group and UUOW group was significantly lower(P0.05).The expressions of MMP-3 and TIMP-1 in UUO group were increased,and the amplitude of increasing expression of TIMP-1 was higher than that of MMP-3.The treatment groups had higher expression of MMP-3(P
