Effect of total flavonoids of Astragalus on apoptosis of cultured bovine retinal capillary pericytes under high glucose
- VernacularTitle:黄芪总黄酮对高糖培养下牛视网膜血管周细胞凋亡的影响
- Author:
Hongyu KUANG
;
Yingying KANG
;
Lili MA
;
Peng DUAN
- Publication Type:Journal Article
- Keywords:
Diabetic retinopathy;
Pericytes;
Total flavonoids of Astragalus;
Apoptosis
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of total flavonoids of Astragalus on apoptosis of bovine retinal capillary pericytes(BRPs) under high glucose.Methods: The third generation of nearly symphysic bovine retinal vessel pericytes cultivated in vitro were divided into normal control group,high glucose group,and Astragalus total flavonoids groups(0.25,0.5,1.0,2.0 mg/ml) at random.After being incubated for 6 days,apoptosis of BRPs were detected by TUNEL method.TBA method was used to detect the contents of MDA.Xanthine oxidase method was used to detect the SOD activities.Results: Compared with high glucose group,the apoptosis,MDA contents,SOD activity,MDA content/SOD activity of BRPs reduced markedly in total flavonoids of Astragalus groups(0.5,1.0,2.0mg/ml)(P
