Generation of differentiated osteoblast specific fgfr1 knockout mice
- VernacularTitle:成熟成骨细胞中敲除基因fgfr1小鼠的制备
- Author:
Fubing LI
;
Ling ZHAO
;
Xiumin LU
;
Ying YU
;
Qifen HE
;
Maomao CHEN
;
Yaqi DUAN
;
Huabing QI
;
Lin CHEN
- Publication Type:Journal Article
- Keywords:
fibroblast growth factor receptor 1;
conditional gene knockout;
mice;
bone development
- From:Journal of Third Military Medical University
1983;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain differentiated osteoblast-specific inactivation of fgfr1 mice Methods To obtain fgfr1△/+/OC-CreTG/+ mice,fgfr1flox/flox mice obtained from NIH were crossed with OC-Cre mice To obtain fgfr1△/△/OC-CreTG/+ mutant mice,fgfr1△/+/OC-CreTG/+ further crossed with themselves or fgfr1flox/flox mice After fgfr1△/△/OC-CreTG/+ crossed with fgfr1flox/flox mice,half of their offspring were mutant mice Results Differentiated osteoblast-specific fgfr1 knockout mice were obtained Conclusion fgfr1△/△/OC-CreTG/+ mice were obtained through proper crossing strategy,which provides a suitable platform for studying fgfr1 function in bone development and fracture healing
