Dorsal root ganglion P2X_3 receptor expression and electrophysiological characteristics in rats with neuropathic pain
- VernacularTitle:神经病理性痛大鼠背根神经节P2X_3受体表达及电生理学特性的改变
- Author:
Xiaohong LIU
;
Junwei ZENG
;
Jinhai ZHANG
;
Huaizhen RUAN
- Publication Type:Journal Article
- Keywords:
nerve injury;
dorsal root ganglion;
P2X receptor;
patch clamp;
ATP
- From:Journal of Third Military Medical University
1983;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the P2X3 receptor expression and electrophysiological characteristics in dorsal root ganglion (DRG) in rat with neuropathic pain caused by chronic constriction injury of sciatic nerve (CCI) Methods P2X3 receptor expressions in L4,L5 and L6 DRG following CCI were observed by using a polyclonal antibody to label the P2X3 receptor ATP-activated currents in corresponding DRG neurons following CCI were observed by using electrophysiological technique Results A significant increase in P2X3 immunoreactivity was observed in the ipsilateral (injured) L4,L5 and L6 DRG and spinal cord on 7,14 d after CCI In small diameter neurons,a significant increase in the number of cells exhibiting a transient current to ATP was observed on 7,14 d after CCI Moreover,amplitude of these currents was increased Conclusion After CCI,the expression and function of P2X3 receptor in corresponding DRG are increased
