Enalaprilat inhibited the proliferation of Neonatal rat Cardiac Fibroblasts via modulation of endothelin-1 and nitric oxide production
- VernacularTitle:依那普利拉通过调控ET-1水平和NO含量抗新生鼠心室成纤维细胞增殖
- Author:
Hongxia SUN
;
Hong LI
;
Shijie YANG
- Publication Type:Journal Article
- Keywords:
enalaprilat;
cardiac fibroblast;
endothelin-1;
nitric oxide;
proliferation
- From:
Chinese Pharmacological Bulletin
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of angiotensinI-converting enzyme inhibitor enalaprilat on proliferation,Endothelin-1 and Nitric oxide System of neonatal cardiac fibroblasts and to probe its mechanism of inhibiting cardiac fibrosis.Methods CFb was isolated by trypsin digestion method.MTT colorimetric assay was adopted to evaluate cell proliferation,HYP method was used to test the collagen synthesis;FCM for cell cycle;Nitric acid reductase method,radio-immunological method was used to detect NO content and ET-1 activity respectively.Results Ena decreased contents of MTT and HYP dramatically.The percentage ratio of phase G0/G1 was increaseded and the percentage radio of phase S was decreased.NO content was increased and ET-1 activity was dropped in a dose and time-dependent manner.Conclusion The anti-proliferative effects of Ena on CFb can be attributed to dropping endothelin-1 activity and enhancing Nitric oxide.
