Effect of Extract of Ginkgo Biloba(EGb)on Cell of Neonatal Rat Cardiocyte Hypertrophy Induced by Prostaglandin F2 Alpha
- VernacularTitle:银杏叶提取物对PGF2?诱导心肌细胞肥大作用的研究
- Author:
Haixia WANG
;
Wenjing DAI
;
Yibo FENG
- Publication Type:Journal Article
- Keywords:
EGb;
Prostaglandin F2 alpha;
Reactive oxygen species;
Cardiocyte hypertrophy
- From:
Journal of Medical Research
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects and mechanisms of extract of ginkgo biloba(EGb)on the cell of cardiocyte hypertrophy induced by prostaglandin F2 alpha.Methods normal cells were used as negative control,and spontaneously PGF2? and EGb were used as experimental groups.The cells were isolated and cultured on cultured neonatal rat cardiocyte by PGF2?,cardiocyte hypertrophy was determined by the cell surface and the total protein of cells;the level of intracellular ROS measured by the fluorescence microscope.Results In cardiocyte hypertrophy,the cell surface,the level of intracellular ROS and the total protein of cells increased significantly in cutured neonatal rat cardiac treated with PGF2?.Compared with cells treated with PGF2?,EGb(40?g/ml,80?g/ml,100?g/ml)inhibited cardiocyte hypertrophy by PGF2?-induced,decreased in the cell surface by 19%,27%,33% and in the total protein of cells by 21%,39%,47% respectively(all P
