Change of Transmembrane Potential During the Early Preconditioning Phase of Noradrenaline's Treatment
- VernacularTitle:去甲肾上腺素预处理早时相线粒体跨膜电位的变化
- Author:
Yuanzhou ZHU
;
Yibai FENG
;
Chao CHANG
;
Yuqin WANG
- Publication Type:Journal Article
- Keywords:
Noradrenaline;
Preconditioning;
Mitochondria;
Transmembrane potential
- From:
Journal of Medical Research
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the change of transmembrane potential during Noradrenaline's early preconditioning phase and probe the mechanism of action.Methods Primary culture myocardial cell of neonate rat were divided into three groups at random.GroupⅠ is control group,Group Ⅱis treatment group by hypoxia,Group Ⅲ is treatment group by NA.Group Ⅰ didn't give any drug to culture for 40minutes;Group Ⅱ treated for 40minutes by hypoxia,Group Ⅲ exposed to Noradrenaline(0.2?g/ml)for 10 minutes then treated with hypoxia for 40 minutes.The fluorescence intensity of rhodamine-123 marked mitochondria were detected by flow cytometry.Results The fluorescence intensity of three Group were test by analysis of variance(F=461.3,P
