Preparation and Characterization of Rabbit Anti-human RBBP_(10) Polyclonal Antibody
- VernacularTitle:兔抗人RBBP_(10)多克隆抗体的制备与鉴定
- Author:
Kan LI
;
Hua LIU
;
Daoming ZHANG
- Publication Type:Journal Article
- Keywords:
Polyclonal antibody;
RBBP_(10)
- From:
Journal of Medical Research
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare rabbit anti-human RBBP10 polyclonal antibody and then character them.Methods New Zealand rabbit was immunized with RBBP10 protein,Polyclonal antiserum was analyzed by ELISA and Western blot.Results The titer of the antiserum obtained in this experiment determined by ELISA was up to 1∶16000 and the sensitivity was at least 10?g/?l.Western blot results show the antibody has high specificity.Conclusions Preparation the Rabbit anti-human RBBP10 Polyclonal antibody in this method is successfully,The expression of the RBBP10 protein in tumor can be detected in immunohistochemistry method,it is useful.
