AQP9 expression in rat cerebral cortex and hippocampus after moderate traumatic brain injury
- VernacularTitle:大鼠轻度脑外伤后水通道蛋白9在大脑皮质及海马的表达变化
- Author:
Hui LIU
;
Shanquan SUN
;
Mei YANG
;
Weihua YU
- Publication Type:Journal Article
- Keywords:
aquaporin-9;
traumatic brain injury
- From:Journal of Third Military Medical University
1983;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expressions of AQP9 and AQP9 mRNA in cerebral cortex and hippocampus of rats after moderate traumatic brain injury(TBI).Methods The moderate TBI model was established according to Feeney's method.At different time points after TBI,the degree of cerebral ischemia by TTC staining,the expressions of AQP9 and its mRNA in brain by immunohistochemistry and RT-PCR respectively were performed.Results No obvious ischemia in brain was found 6 h after TBI,but gradually the ischemic focus enlarged,and reached the maximum 72 h after TBI.The expressions of AQP9 and its mRNA were similar,increasing significantly 3 h after TBI,decreasing 6 h after TBI,then increasing again at 24 h in bilateral cerebral cortex and the hippocampus and reaching the maximum 48 h or 72 h after TBI.Conclusion The increased expression of AQP9 after TBI is involved in the stress of early TBI and in later brain energy metabolism.
