Effect of Isoflurane on ICAM-1 expression in rats during liver ischemia-reperfusion injury
- VernacularTitle:异氟烷对大鼠肝脏缺血再灌注损伤时细胞间黏附分子-1表达的影响
- Author:
Guangmin XU
;
Guocai TAO
;
Bin YI
- Publication Type:Journal Article
- Keywords:
Isoflurane;
ischemia-reperfusion injury;
intercellular adhesion molecule-1
- From:Journal of Third Military Medical University
1983;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of intercellular adhesion molecule-1(ICAM-1)in Isoflurane against hepatic ischemia-reperfusion(I/R)injury in vivo.Methods Thirty-two female SD rats were randomly divided into 4 groups:sham-operation group(A),I/R group(B),Isoflurane group(C),I/R+ Isoflurane group(D).Groups A and C were be used as controls.In groups B and C,the hepatic hilar vessels distributing to the left and median lobes were clamped to induce partial hepatic ischemia(70%).Reperfusion was done 60 min after hepatic ischemia.The animals were killed 3 h after reperfusion,then the specimens of liver tissues and blood were obtained.ALT and AST in blood serum were detected as liver damage markers.The histological examination of liver tissues was done by hematoxylin and eosin staining.The levels of ICAM-1 protein in the hepatic tissues were detected by immunohistochemistry.Results After hepatic ischemia-reperfusion,the ICAM-1 protein expression in the hepatic tissues increased;ALT and AST increased;Reperfusion induced severe liver damage.Isoflurane significantly inhibited the ICAM-1 expression.Conclusion The degree of hepatic ischemia-reperfusion injury is closely associated with the expression level of ICAM-1.Isoflurane abates liver ischemia-reperfusion injury and inhibits the ICAM-1 expression.
