THE SYNERGISTIC EFFECTS OF HERCEPTIN AND 9-CIS-RETINOIC ACID ON HER2/NEU POSITIVE BREAST CANCER CELLS IN VIVO
- VernacularTitle:赫赛汀和9-顺式视黄酸联合用药对裸鼠转染HER2基因乳腺癌效应的研究
- Author:
Haibin LANG
;
Mantian MI
- Publication Type:Journal Article
- Keywords:
Herceptin;
9-cis-RA;
breast cancer;
HER2;
nude mice
- From:
Acta Nutrimenta Sinica
1956;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the synergistic effects of Herceptin(HER)and 9-cis-retinoic acid (9-cis-RA) on HER2/neu positive breast cancer cells in vivo. Method MCF-7 breast cancer cells transfected with exogenous HER2 gene were inoculated into athymic nude mice. Four weeks after tumor inoculation,mice bearing a tumor of 200 mm3 in volume approximately were selected to be treated with HER and/or 9-cis-RA(SC) for 20d. After that tumor volume was precisely measured,concurrently the HE staining and Ki-67 immunohistochemical (IHC) assay were performed to detect the anti-proliferative effect of HER in combination with 9-cis-RA in vivo. Results After 3 w of treatment,HER and 9-cis-RA synergistically decreased the tumor volume as compared with control group. IHC results showed that Ki-67 expression was significantly inhibited by co-treatment of HER and 9-cis-RA,which means the arrest of cell cycle progression. Conclusion Herceptin and 9-cis-RA could synergistically inhibit the proliferation of HER2 positive breast cancer cells in vivo.
