Screening the target genes of Schistosoma japonicum related to the naturely resistance of Microtus fortis
- VernacularTitle:东方田鼠抗日本血吸虫抗性相关靶基因筛选
- Author:
Yi SUN
;
Huan SUN
;
Renchu JIA
;
Jinming LIU
;
Chunxiu YUAN
;
Yaojun SHI
;
Ke LU
;
Hao LI
;
Yamei JIN
;
Jiaojiao LIN
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Microtus fortis;
Phage display cDNA library;
Target gene;
Biopanning
- From:
Chinese Journal of Schistosomiasis Control
1989;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To look for the genes of Schistosoma japonicum related to the Schistosoma-resistance of Microtus fortis.Methods The fresh sera of Microtus fortis were used to screen a T7 phage display cDNA library from worms of Schistosoma japonicum established in our lab.The positive clones were sequenced and functionally analysed through bioinformatics.Results The specific phages binding to the sera of Microtus fortis were enriched 857-fold after three rounds of biopanning,and 58 positive clones picked at random were sequenced and 10 ESTs were obtained.BLASTn results showed that 7 ESTs had 99%-100% similarity to the genes of Shistosoma japonicum reported in GenBank and 1 EST had 82% similarity to a zinc finger protein encoden gene from Pan troglodytes.The results of these ESTs function prediction indicated most of them were involved in the regulation of gene expresion of Schistosoma japonicum.Conclusions Several target genes of Schistosoma japonicum related to the Schistosoma-resistance of Microtus fortis are obtained and those would lay foundation to expatiate the native resistance mechnism of Microtus fortis to Schistosoma japonicum.
