Effects of Naomaitong on regulateing gene expression of gelatinase system after cerebal ischemia/reperfusion in aged rats
- VernacularTitle:脑脉通对老龄大鼠脑缺血/再灌注明胶酶系基因表达调节作用的影响
- Author:
Ke LIU
;
Jiansheng LI
;
Minghang WANG
;
Jingxia LIU
;
Yuewu ZHAO
;
Zhengguo LIU
- Publication Type:Journal Article
- Keywords:
Cerebral ischemia/reperfusion;
Cerebro-microvessel basement membrane;
Gelatinase system;
Old age;
Naomaitong;
Experimental study
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study effects of Naomaitong on injury of cerebro-microvessel basement membrane in different periods and gene expression of gelatinase system after cerebral ischemia/reperfusion(I/R) in rats.Methods: Focal cerebral ischemia/reperfusion model was established by MCAO method of the intraluminal filament technique.Rats were divided into sham-operated group,model group,Naomaitong group and Nimodipine group at random,the later three groups with ischemia 3h and I/R 6h,12h,24h,3d,6d.Ransmission electron microscope and hybridization in situ method were used to observe the structure of micrangium and gene expression of gelatinase and its inhibitor.Results: Under electron microscope,the pathodamage in medico-group seemed lighter.According to the gene expression of gelatinase system,Naomaitong could step down the mRNA expression of MMP-2(I/R 6h-6d)and MMP-9(I/R 12h,I/R 3d).And increase the mRNA expression of TIMP-1(I/R 6h-3d).Conclusion: The protection of Naomaitong on cerebro-microvessel basement membrane after cerebral I/R in rats are related to the gene expression regulation of gelatinase system.
