Effects of FuzhengJiedu decoction on antioxidant enzyme activity and MDA contents of human bronchial epithelial cells exposed to NiSO_4
- VernacularTitle:扶正解毒汤对染镍体外培养人支气管上皮细胞抗氧化酶活性及丙二醛含量的影响
- Author:
Xiaoyun LV
;
Yuzhen ZHU
;
Yulian TENG
;
Xiaoli PU
- Publication Type:Journal Article
- Keywords:
Fuzheng Jiedu decoction;
Nickel sulfate;
Human bronchial epithelial cells;
Glutathione peroxidase(GSH-Px);
Superoxide dismutase(SOD);
Maleic dialdehyde(MDA)
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of pharmaco-serum from rabbits administrated intragastrically with Fuzheng Jiedu decoction(FJD) on injured human bronchial epithelial(16HBE) cells exposed to nickel sulfate(NiSO4).Methods: Cultured 16HBE cells were treated with both NiSO4 and different doses of FJD pharmaco-serum in vitro.The detections of cell activities and viabilities were carried out.Meanwhile,activities of glutathione peroxidase(GSH-Px) and superoxide dismutase(SOD) as well as maleic dialdehyde(MDA) contents were measured.Results: FJD pharmaco-serum could decrease mortalities and increase viabilities of 16HBE cells exposed to NiSO4.In cells co-treated with NiSO4 and FJD pharmaco-serum,the content of MDA was decreased,while GSH-Px and SOD activities were increased at the same time.High dose of FJD pharmaco-serum had the most dramatic effect.Conclusion: This study suggested that FJD could antagonize NiSO4-induced toxicity,which may be involved in its antioxidant function.
