RP-HPLC Method Measure the Contents and The Regularity of Rhychophyline in Ramulus Uncariae Cum Uncis
- VernacularTitle:RP-HPLC法测定钩藤煎剂中钩藤碱含量及变化
- Author:
Qinghua LIU
- Publication Type:Journal Article
- Keywords:
Ramulus Uncariae Cum Uncis;
Rhychophyline;
RP-HPLC
- From:
Journal of Zhejiang Chinese Medical University
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To measure the contents of rhychophyline in uncaria,and its regularity of variety with decoction time.[Methods]RP-HPLC method was used.The chromatographic conditions were listed below:Hypersil-BDS C18 column(150mm?4.6mm.5?m),amobine phase composed of metranol-water(70∶30),a flow rate of 1ml /min and wave lengths was 254nm,column temperature was 25 ℃.[Results]In the range of 0.19?g ~0.95?g,the linear was very well between the content of rhychophyline and peak area.[Conclusion]The contents of rhychophyline in decoction reached the peak at 11.4min after uncaria was added in boiling water.
