Comparison on the antineoplastic immunological effects of nanoemulsion vaccine given throngh different routes
- VernacularTitle:纳米疫苗经不同途径给药后的抗肿瘤免疫学效应比较
- Author:
Wei GE
;
Yuan LI
;
Shanhong ZHANG
- Publication Type:Journal Article
- Keywords:
nanotechnology;
tumor vaccine;
drug administration routes
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the antineoplastic immunological effects of nanoemulsion-encapsulated MAGE1-HSP70 and SEA(the ratio of MAGE1-HSP70 fusion protein to SEA superantigen was 100∶1)as NE(MHS)vaccine as given in different routes,and try to look for a novel and effective immune route.Methods NE(MHS)was prepared using magnetic ultrasound methods,and the size,the encapsulation rate and the stability of the nanoemulsion vaccine were evaluated.C57BL/6 mice were immunized via p.o.,subcut.,i.v.,or i.p.route.The cellular immunocompetence was detected by ELISpot assay and LDH release assay.The therapeutic and tumor challenge assay were examined too.Results When the vaccine was given orally,the tumor masses formed 28 days after B16-MAGE1 inoculation in mice were markedly bigger than that formed in the mice of the other groups(P
