Nanobacteria: isolation from prostatic calculus and identification of its 16S rRNA gene
- VernacularTitle:前列腺结石患者结石中纳米细菌的分离和16S rRNA基因的鉴定
- Author:
Xuecheng SHEN
;
Jie YANG
;
Xiancai RAO
;
Bo SONG
;
Zhansong ZHOU
- Publication Type:Journal Article
- Keywords:
nanobacteria;
prostatic calculus;
polymerase chain reaction;
16S rRNA
- From:Journal of Third Military Medical University
1984;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the distribution of nanobacteria in prostatic calculus and investigate its role in the formation of prostatic calculus. MethodsThe stones of 40 patients with prostatic calculus was used to isolate and culture the possible bacteria. The genomes of obtained bacteria were extracted, and the 16S rRNA was amplified by PCR followed by sequencing. ResultsThe obtained specific fragment had a 98% resemblance with 16S rRNA of nanobacteria: Score=2 480 bits (1 290), Expect=0.0; Identities=1 387/1 409 (98%), Gaps=4/1 409 (0%); Strand=Plus/Plus. ConclusionNanobacteria is proved existing in the stones of prostatic calculus patients by PCR and sequencing.
