Effect of TNF-? on blood-labyrinth barrier permeability of microvascular endothelial cells from cochlear stria vascularis of guinea pig
- VernacularTitle:TNF-?对体外培养豚鼠耳蜗血管纹微血管内皮细胞通透性的影响
- Author:
Qi LI
;
Xueyuan ZHANG
- Publication Type:Journal Article
- Keywords:
TNF-?;
blood-labyrinth barrier;
endothelial cells;
permeability
- From:Journal of Third Military Medical University
1984;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the mechanism of TNF-? on regulating the blood-labyrinth barrier permeability of cochlea of guinea pig.MethodsThe stria vascularis was acquired from guinea pigs and the endothelial cells were cultured of with purified to establish the models of the blood-labyrinth barrier according to our previous researches.The models were treated with TNF-?(0.05,0.1,0.2 ng/ml)for 30,60,90 min or with serum-free DMEM as control.Then their permeability was measured by Evens blue technique.ResultsTNF-? increases Evans blue transfer across endothelial cell monolayer(P
