The effect of breviscapine on pulmonary fibrosis induced by bleomycin in rats
- VernacularTitle:灯盏花素对实验性肺纤维化大鼠的干预作用
- Author:
Wenshu CHAI
;
Shengping ZHAI
;
Sulin WU
;
Baiyi CHEN
- Publication Type:Journal Article
- Keywords:
breviscapine;
pulmonary fibrosis;
apoptosis;
Fas/FasL;
oxygen free radical
- From:
Chinese Pharmacological Bulletin
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the expressions of Fas/FasL and apoptosis of pulmonary tissue as well as oxygen free radical in the pulmonary fibrosis induced by bleomycin in rats at inflammation stage and fibrosis stage.Methods Forty-eight rats were divided into three groups randomly,including control group,model group and breviscapine group.On experimental day 0,the rats were intratracheally instilled with bleomycin,and then treated with breviscapine 10 mg?kg-1?d-1 until they were killed.On days 7,28 after stillation,eight rats of each group were killed and the lungs were harvested for examination.Flow cytomagnetometry was used to detect the changes of cell apoptosis and the expressions of Fas/Fasl in rats with pulmonary fibrosis caused by bleomycin at flammation stage and fibrosis stage.Malonaldehyde and hydroxyproline(HYP)of lung tissue were detected by TAB method.Results The apoptosis index of lung cells in the pulmonary fibrosis group was higher than that of control and breviscapine group.The expression of Fas/FasL and contents of Malonaldehyde as well as hydroxyproline in the pulmonary fibrosis group were higher than those of control group and breviscapine group(P
