Effects of thrombospondin-1 typeⅠ repeat antisense RNA on human endothelial cells
- VernacularTitle:凝血栓蛋白-1Ⅰ型重复序列反义核酸在血管内皮细胞中的作用研究
- Author:
Xiaowei LI
;
Yili LIU
;
Pingsheng WU
;
Zhongjiang ZHOU
;
Ping LIU
- Publication Type:Journal Article
- Keywords:
thrombospondin-1 typeⅠrepeat;
endothelial cell;
cell proliferation;
antisense RNA
- From:
Chinese Pharmacological Bulletin
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of thrombospondin-1 type Ⅰ repeat antisense RNA on cell proliferation and growth activity of human endothelial cells.Methods The eukaryotic expression vector of pcDNA3.1-/anti-TSP-1-Ⅰ was constructed.Then the vector was transfected into human umbilical vein endothelial cells(HUVECs).The growth activity of endothelial cells after transfection was detected by MTT.The variation of endothelial cell's cycle was determined by flow cytometry.Cell morphological change was observed by transmission electron microscope.Results The eukaryotic expression vector(pcDNA3.1-/anti-TSP-1-Ⅰ)was successfully constructed and transfected into HUVECs.The OD value of pcDNA3.1-/anti-TSP-1-Ⅰ transfective group was promoted compared with that of nontransfective group(P
