A method for detection of HER2 gene amplification in breast cancer tissues:chromogenic in situ hybridization method and its modification
- VernacularTitle:乳腺癌HER2基因扩增检测方法——显色原位杂交法的应用及优化
- Author:
Canrong NI
;
Chenguang BAI
- Publication Type:Journal Article
- Keywords:
chromogenic in situ hybridization;
breast neoplasms;
human epidermal growth factor receptor 2;
c-erbB-2 gene
- From:
Academic Journal of Second Military Medical University
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To apply chromogenic in situ hybridization(CISH)for detection of HER2 gene amplification in breast cancer tissues and to discuss some modifications of the CISH method.Methods:HER2 gene amplification was detected by CISH in 60 breast cancer specimens with an immunohistochemical score over 2+.The correlation between the results of IHC and CISH was analyzed.Our experience in CISH manipulation was summarized and optimization to CISH was discussed.Results:CISH identified gene amplification in 91%(40/44)specimens with an IHC score of 3+ and in 50%(8/16)specimens with an IHC score of 2+.The total concordance rate between IHC and CISH was 80%(48/60,P
