Determination the Content of Paeoniflorin in Anwei Granules by HPLC Method.
- VernacularTitle:高效液相色谱法测定安胃颗粒中芍药苷的含量
- Author:
Lin TIAN
;
Haiyang HAN
- Publication Type:Journal Article
- Keywords:
HPLC;
Paeoniflorin;
Anwei Granules
- From:
Journal of Medical Research
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method for determination of the content of paeoniflorin in in Anwei Granules.Methods With Phonomenex C18,150mm?4.60mm,5micron,acetonitrile-water-phosphoric acid(15∶85∶0.2)was used as the mobile phase,The flow rate was 1.0ml/min.The detection wavelength was 230nm.Results The linearity was obtained from 2.52-20.14 ?g(r=0.9990).The average recovery was 99.17 % with RSD=0.91%(n=6).Conclusions The method is sensitive,simple,specific and accurate for determination of astragaloside in Tangshenkang Granules.
