Effects of a new recombinant fusion protein rTMP-GH on proliferation of megakaryocytes in vitro
- VernacularTitle:重组融合蛋白rTMP-GH对体外培养巨核细胞增殖的影响
- Author:
Yang XU
;
Junping WANG
;
Jinghong ZHAO
;
Yongping SU
;
Fang CHEN
;
Mingqiang SHEN
;
Mo CHEN
;
Song WANG
;
Li KAI
- Publication Type:Journal Article
- Keywords:
thrombopoietin mimetic peptide-growth hormone;
Dami cells;
Proliferation;
GATA-1
- From:Journal of Third Military Medical University
1984;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of rTMP-GH, a recombinant fusion protein of thrombopoietin mimetic peptide (TMP) and human growth hormone (GH), on the proliferation and thrombocytopoiesis of cultured megakaryocytes. Methods After being treated with 100 ng/ml of rTMP-GH for 7 d, Dami cells, a kind of megakaryocyte cell line, were analyzed by observing the numbers of colony forming unit. Meanwhile, Western blotting and RT-PCR were applied to detect the expression changes of globin transcription factor-1 (GATA-1), which is a main regulator of thrombocytopoiesis in megakaryocytes. Results The numbers of colony forming unit were markedly increased in cultured Dami cells incubated with rTMP-GH. Compared with normal control and GH treatment groups, both the mRNA and protein levels of GATA-1 were up-regulated significantly in Dami cells treated with rTMP-GH. Conclusion rTMP-GH has a strong ability to promote the proliferation and thrombocytopoiesis of megakaryocytes.
