Tissue engineering of dentin-pulp complex-like structures by rat first branchial arch cells
- VernacularTitle:大鼠第一腮弓细胞构建组织工程牙本质-牙髓复合体样结构
- Author:
Manjing DENG
;
Xiaohong CHEN
;
Luchuan LIU
;
Jianping AN
;
Guiying KUANG
- Publication Type:Journal Article
- Keywords:
the first branchial arch cells;
dentin-pulp complex;
odontogenesis
- From:Journal of Third Military Medical University
1984;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the odontogenetic ability of first branchial arch cells of E12.5 rats. Methods First branchial arch cells (mandibular process) of E12.5 SD rats were isolated enzymatically and collected. After combined with gelatin sponge, the cells were transplanted into the renal capsule of a rat. The specimen was taken out and evaluated by histological and immunohistochemical methods in 4 weeks after growth in renal capsule. Results The first branchial arch cells with gelatin sponge developed dentin-pulp complex-like structure. Dentin sialoprotein (DSP) was expressed in the newly formed dentin-like structure. The green mineralized matrix was further identified with Masson’s trichrome staining. Conclusion Cells from first branchial arch of E12.5 rats can partially keep genetic signal of tooth growth and form dentin-pulp complex-like structure in renal capsule.
