Effect of ambroxol on the structure of Pseudomonas aeruginosa biofilm
- VernacularTitle:氨溴索对铜绿假单胞菌生物膜结构的影响
- Author:
Fang LI
;
Jialin YU
;
Guanxin LIU
;
Hua YANG
- Publication Type:Journal Article
- Keywords:
ambroxol;
Pseudomonas aeruginosa;
biofilm
- From:Journal of Third Military Medical University
1984;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of ambroxol on the structure of Pseudomonas aeruginosa biofilm (BF) in an established biofilm model in vitro. Method Mature BF was formed after 7 days′ culture of Pseudomonas aeruginosa on plate. The BF structure was observed by scanning electron microscope (SEM). The parameters of BF structure were analyzed though pictures from confocal laser scanning microscopy (CLSM) with Image Structure Analyzer (ISA) software. Fluorescence intensity of the viable bacteria in BF treated by ambroxol alone and together with ciprofloxacin were determined with fluorescence microscopy. Results After the treatment of ambroxol, the BF was destroyed and the matrix outsides became thin and abnormal compared with the control by SEM, and small amount of bacteria dispersed. The results of ISA showed that with the treatment of 2 mg/ml ambroxol, BF was decreased in thickness, average diffusion distance (ADD) and textual entropy (TE) were decreased in comparison with the group without ambroxol treatment (P
