Effects of Pine Pollen on Expression of p16 and p21 in Human Embryonic Lung Diploid Fibroblasts
- VernacularTitle:松花粉对人胚肺二倍体成纤维细胞p16及p21基因表达的影响
- Author:
Xin ZHONG
;
Lu YU
- Publication Type:Journal Article
- Keywords:
pine pollen;
cell aging;
cell cycle;
P16;
P21
- From:
Journal of Zhejiang Chinese Medical University
2007;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To observe the effects of pine pollen on the changes of p16INK4a and p21WAF1 in 2BS cells.[Method]The 2BS cells were cultured from 28 population doubling(PD)and divided equally into the young control group,old control group,middle-dose pine pollen group(120mg/dl)and high-dose pine pollen group(240mg/dl)randomly.Regulate the phases of cell cycle by flow cytometry,and then the expression of p16INK4a,p21WAF1 mRNA were determined by semi-quantitative RT-PCR.[Result]The amount of cells in G1 phase was depressed in middle-dose pine pollen group(72.73%?1.76%)and high-dose pine pollen group(64.15%?0.87%)than in old control group(81.33%?3.43%)noticeablely(P
