Study on quick extraction and determination of icariin from Herba Epimedii
- VernacularTitle:淫羊藿中淫羊藿苷的快速提取及测定研究
- Author:
Ning JIANG
;
Xiaopeng LIU
- Publication Type:Journal Article
- Keywords:
Icariin;
Extraction;
RP-HPLC;
Content
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the quick extraction and determination of icariin in Herba Epimedii.Methods: The orthogonal design and ASE300 system were used to study the extraction method of icariin.The content of icariin was determined by HPLC.The methanol water glacial acetic acid(60∶40∶0.25) was used as a mobile phase. The flow rate was 1.0ml/min,the detection wavelength was 270nm and the column temperature was 41℃.Results: The optimal extraction conditions were: 50% alcohol and extracting one times for twenty minutes each time at 70℃.The extracting rate of icariin was 93.15%.The RP-HPLC for determining method is simple,rapid and accurate.Conclusion: The extraction and determination methods are practicable for icariin in Herba Epimedii.
