Effects of centipede acidic protein on the proliferation and collagen synthesis of cultured neonatal rat cardiac fibroblast induced by angiotensinⅡ
- VernacularTitle:蜈蚣酸性蛋白对血管紧张素-Ⅱ诱导心肌成纤维细胞增殖和胶原合成的影响
- Author:
Zhiguo ZHAO
;
Shengjiang GUAN
;
Wei ZHANG
;
Junyun LI
;
Lin WANG
;
Ye JIANG
;
Li CHU
- Publication Type:Journal Article
- Keywords:
Centipede Acidic Protein;
Cardiac fibroblast;
Angiotensin;
Collagen;
c-myc;
Experimental study
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the influence of Centipede Acidic Protein(CAP) on the proliferation and collagen synthesis of cultured neonatal rat cardiac fibroblasts(CFb) induced by angiotensinⅡ(AngⅡ),and to explore the mechanisms of CAP on cardiac fibrosis.Methods: Neonatal rat cardiac fibroblasts were treated with AngⅡ to produce fibrosis model.The effects of CAP on proliferation of CFb were observed by MTT colorimetric assay,synthesis of collagen was observed by the hydroxyproline concentration.The NO contents were measured by Nitric acid reductase method.The c-myc expression was examined by semi-quantitative RT-PCR analysis.Results: Compared with that of control group,the proliferation,collagen synthesis and the levels of c-myc mRNA expression of CFb in the model group increased,while the NO contents decreased obviously(P
