Effects of peritoneal dialysate on the expression of aquaporin-1 in rat peritoneal mesothelial cells
- VernacularTitle:腹透液对大鼠腹膜间皮细胞水通道蛋白1表达的影响
- Author:
Shengxiao CHEN
;
Hao REN
;
Zhengrong LIU
- Publication Type:Journal Article
- Keywords:
dialysis solutions;
peritoneal dialysis;
peritoneal mesothelial cells;
aquaporin
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the effects of peritoneal dialysate (PDS) on the expression of aquaporin-1 in rat peritoneal mesothelial cells (RPMCs). Methods The in vitro cultured RPMCs were divided randomly into 4 groups, and stimulated respectively with 1640 culture medium containing 10% fetal calf serum (negative control), and three kinds of PDS (containing respectively 4.25% glucose, 4.25% mannitol or 1.5% glucose) for 3 hours. Indirect immunofluorescence assay (IFA) and flow cytometry (FCM)were employed to detect the expression of aquaporin-1 in RPMCs stimulated by different kind of PDS as above. Results Compared with negative control and the group with PDS containing 1.5% glucose, the expression of aquaporin-1 on the mesothelial cells of the group with PDS containing 4.25% glucose and that containing 4.25% mannitol was significantly up-regulated (P0.05). Conclusion PDS with higher osmotic pressure (OP) can enhance the expression of aquaporin-1 in RPMCs. For PDS with same OP (PDS containing 4.25% glucose or PDS containing 4.25% mannitol), those containing glucose can enhance the expression of aquaporin-1 more effectively than that PDS containing mannitol. This result indicates that glucose or its degradation products may be the independent factors in enhancing the expression of aquaporin-1 of RPMCs.
