Changes of von Wilebrand factor in rabbits with acute lung injury and the protective effects of heme oxygenase
- VernacularTitle:兔急性肺损伤vWF变化及血红素加氧酶的保护作用
- Author:
Lina GUO
;
Jianxin WANG
;
Qiang GAO
- Publication Type:Journal Article
- Keywords:
acute lung injury;
Von Willebrand factor;
heme oxygenase-1;
hemin
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes in concentration of plasma von Willebrand factor (vWF) and the effects of heme oxygenase-1 (HO-1) in rabbits with endotoxin (ET)-induced acute lung injury (ALI). Methods Twenty four male New-Zealand rabbits were randomly assigned to three groups: control group, ET group and hemin-treatment group. Saline was iv. injected into the animals of control group; in animals of ET-treatment group ALI model was reproduced with endotoxin iv. injection (700 ?g/kg); animals of hemin-treatment group received intraperitoneal injection of heme for 2 days before ET challenge (40?mol/kg, 2 times per day). Arterial blood gas assay was performed and the plasma vWF concentration was determined with ELSIA at 0h (pre-experiment), 0.5, 1, 2 and 4h time points for the animals in all three groups. When the rabbits were sacrificed, the dry/wet weight ratio of lung and HO-1 (mean density) were determined and the pathological changes in lung tissue were examined. Results Compared with control group, the partial pressure of oxygen (PaO2) and oxygenation index (PaO2/FiO2) declined, meeting the diagnostic criteria of ALI, and plasma vWF concentrations increased obviously (P
