Effect of midazolam on amino acid levels in cultured PC12 cells challenged by N-methyl-D-aspartate
- VernacularTitle:咪达唑仑对N-甲基-D-天冬氨酸致伤PC12细胞氨基酸水平的影响
- Author:
Henglin WANG
;
Zhuoqiang WANG
;
Xiuzhen LIU
- Publication Type:Journal Article
- Keywords:
midazolam;
excitatory amino acids;
N-methylaspartate;
PC12 cells
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of midazolam (MID) on amino acid levels in cultured PC12 cells challenged by N-methyl-D-aspartate (NMDA), and to explore the possible protective action of midazolam which is an anesthetic. Methods Cultured PC12 cells were divided into control group, NMDA group, and MID group. In NMDA group, PC12 cells were challenged by 300?mol/L NMDA in vitro. In MID group, 3?mol/L or 30?mol/L of MID was added to the challenged PC12 cell culture, thus forming two subgroups. After being treated with NMDA 300?mol/L for 4 hours, the PC12 cells were collected, rinsed, levigated and centrifuged (12 000r/min for 20min, at 4℃), then the supernatant liquid was collected. The levels of amino acids were determined with high performance liquid chromatograpy (HPLC). Results After exposing to NMDA 300?mol/L for 4 hours, the level of glutamate released from PC12 cells rose significantly, whereas the level of glutamine, aparatate and glycine remained unchanged. In the presence of MID 3?mol/L and 30?mol/L for 4 hours, the level of glutamate was lowered significantly (P
