The effect of Tanshinone IIA on the expression of NF-?B、I?B-? and the mRNA of ICAM-1 and VCAM-1 of ECV304 induced by TNF-?
- VernacularTitle:丹参酮ⅡA对TNF-?诱导的ECV304细胞NF-?B、I?B-?表达及粘附分子ICAM-1、VCAM-1mRNA表达的影响
- Author:
Guangzhi CUI
;
Shumei JIN
;
Guifeng ZHAO
;
Yingchang FAN
- Publication Type:Journal Article
- Keywords:
tanshinone IIA;
ECV304;
NF-?B;
adhesion molecule;
mRNA
- From:
Chinese Pharmacological Bulletin
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To illustrate the role of Tanshinone IIA(TanIIA)extracted from dan-shen root in atherosclerosis and its different targets by investigating the effect on the expression of NF-?B、I?B-? and the mRNA of ICAM-1 and VCAM-1 in human umbilical vascular endothelial cell 304(ECV304).Mehods The TNF-?-induced cultured ECV-304 cell injury model was established to analyze the expression of NF-?B、I?B-? by cell ELISA method and the mRNA of ICAM-1 and VCAM-1 by RT-PCR method of the cells cultured with different denses of TanIIA.Results The results of ELISA method showed that the expression of the NF-?B was reduced significantly and the expression of the I?B-? was enhanced significantly in ECV304 which were cultured with higher denses of TanIIA.The results of RT-PCR method showed that the mRNA of ICAM-1 and VCAM-1 induced by TNF-? was significantly refrained.Conclusions TanIIA has a significant effect on inhibiting the mRNA of ICAM-1 and VCAM-1 induced by the NF-?B activation,so it can play an impotant role in resisting atherosclerosis.
