Construction of PSA and NCAM differently expressing COS-7 cell line and the effects of PSA on migration and invasion of the diverse COS-7 cell
- VernacularTitle:PSA及NCAM表达差异COS-7细胞的建立及PSA对COS-7细胞迁移侵袭能力的影响
- Author:
Yabin CHENG
;
Jing LI
;
Gong DAI
;
Meiyu GENG
- Publication Type:Journal Article
- Keywords:
PSA;
NCAM;
COS-7;
migration;
invasion;
cancer
- From:
Chinese Pharmacological Bulletin
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To construct a polysialic acid(PSA)and neural cell adhesion molecule(NCAM)differently expressing COS-7 cell line,and investigate the effects of PSA on the cell adhesion,migration and invasion,aiming to establish the base for further investigation of the signaling passway of the diverse celluar migration and invasion,and elucidate the molecular mechanism of PSA promoting cancer cell metastasis.Methods A polysialic acid and neural cell adhesion molecule differently expressing COS-7 cell line was constructed by transient cotransfection,and the cotransfection efficiency was determined by Western blot and flow cytometry.Adhesion assay was used to investigate the effect of PSA on cell adhesion ability;transwell assay was used to measure migration and invasion ability.Results The PSA and NCAM differently expressing COS-7 cell line was successfully constructed,which demonstrated PSA inhibited the cell adhesion to basement membrane,and promoted the migration and invasion ability.Conclusions The constructed polysialic acid and neural cell adhesion molecule differently expressing COS-7 cell line can be used to investigate molecular mechanism of promoting cancer cell metastasis induced by PSA in the future.
