A mechanism study of the inhibition of the adhesion of human gastric cancer MKN1 cells and suppression of the expression of integrin ?_1 gene by dextran sulfate
- VernacularTitle:硫酸右旋糖苷抑制人胃癌细胞粘附以及整合素?_1基因表达的机制研究
- Author:
Yuanyi XU
;
Yunning HUANG
;
Wei WANG
;
Jingxian MA
;
Jianda DONG
;
Xiangmei CAO
;
Lin ZHAO
;
Yong LIU
;
Hong GAO
- Publication Type:Journal Article
- Keywords:
dextran sulfate;
human;
gastric cancer;
integrin ?_1
- From:
Chinese Pharmacological Bulletin
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the adhesive process of the human gastric cancer cell line MKN1,and study the expression of integrin ?1;to investigate the mechanism of the inhibition of the adhesion process of MKN1 cells by destran sulfate(DS).Methods The MKN1 cells were cultured with DS or PBS,then stained with immunofluorescent cytochemistry and observed in fixed or living conditions with confocal laser scanning microscope.RT-PCR was used to analyze the cDNA expression of MKN1 cells.Results MKN1 cells adhered to culture dishes by the process of forming filopodia,changed into a flat shape,and then adhered to other cells to form a cell-monolayer.Integrin ?1 was intensively expressed in the cell membrane,where integrin ?1 formed clusters.DS inhibted the expression of integrin ?1 in cell membrane,and decreased the area of integrin ?1 clusters.DS-treated cells also tended to maintain a round shape by contracting the filopodia.In DS-containing culture dishes,some cells kept floating 4 hours after seeding.DS decreased the level of the cDNA expression of the adhered cells to 74% and of the floating cells to 38% of that of the cells in un-treated group,respectively.Conclusion The inhibition of the adhesion of MKN1 cells by DS was related to the suppression of the expression of integrin ?1.
