Effect of hydrogen sulfide on oxidative stress in hypoxic pulmonary hypertension
- VernacularTitle:硫化氢对低氧性肺动脉高压中氧化应激的调节作用
- Author:
Hongling WEI
;
Junbao DU
;
Chaoshu TANG
;
Chunyu ZHANG
;
Hongfang JIN
;
Qin SI
;
Yue TIAN
- Publication Type:Journal Article
- Keywords:
Hydrogen sulfide;
Hypertension, pulmonary;
Oxidative stress;
Anoxia
- From:
Journal of Peking University(Health Sciences)
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the modulatory effect of hydrogen sulfide (H2S) on oxidative stress in the development of pulmonary hypertension induced by hypoxia. Methods:Twenty male Wistar rats were randomly divided into control group (n=6), hypoxic group (n=6) and hypoxia+NaHS group (n=8). Hypoxic challenge was performed everyday for 21 days. NaHS solution was injected intra-peritoneally everyday before hypoxic challenge for rats in the hypoxia+NaHS group. After 21 days of hypoxia, the mean pulmonary artery pressure(mPAP) was measured by cardiac catheterization. The weight ratio of right ventricle to left ventricle+septum [RV/(LV+SP) ] was also measured. The lung homogenates were assayed for total antioxidant capacity(T-AOC), superoxide dismutase (SOD), oxidized glutathione (GSSG), reduced glutathione (GSH), malondiadehyde(MDA) and hydroxy radical(?OH), and the SOD mRNA levels were assayed by real time polymerse chain reaction. Results: After three weeks of hypoxic disposure, hypoxic hypertension and vascular remodeling developed. Compared with the control group, the mPAP[(23.7?2.2) mm Hg vs. (16.3?3.7) mm Hg,P
