Influence of N-acetylcysteine on Pseudomonas aeruginosa biofilm
- VernacularTitle:N-乙酰半胱氨酸对铜绿假单胞菌生物被膜影响的实验研究
- Author:
Wei XIE
;
Jie MENG
;
Chengping HU
- Publication Type:Journal Article
- Keywords:
Acetylcysteine;
Levofloxacin;
Pseudomonas aeruginosa;
Biofilm
- From:
Chinese Journal of Practical Internal Medicine
2006;0(24):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of N-acetylcysteine(NAC)on the formation of Pseudomonas aeruginosa biofilm(BF),and to study the synergism of bactericidal activities of NAC combined with levofloxacinon to the mature Pseudomonas aeruginosa biofilm.Methods The experiment was done in experiment centre of Xiangya Hospital.Scanning electronic microcopy(SEM)were utilized to demonstrate the effect of NAC on the formation of biofilm.Then biofilm cultured without NAC were detected by optical microscopy after being stained with AgNO3.The minimal inhibitory concentration were detected by double test tube diluted method.The mature biofilm was treated by NAC and/or different concentrations of levofloxacin for 24 hours.The influence of levofloxacin combined with NAC were examined by MTT method on the bacterial quantity in biofilms.Results Biofilm was achieved on the silicon slides in all groups 7 days after the incubation of Pseudomonas aeruginosa.Scanning electronic microcopy showed that the mucoid materials among bacteria was significantly reduced and the thickness of biofilm was decreased in NAC groups.Levofloxacin beyond 1 MIC could significantly decline the viable cells on biofilms(P
