Effects of spironolactone on expression of ?1(Ⅰ) and ?1(Ⅲ) procollagen mRNA collagen in rat hepatic fibrosis.
- VernacularTitle:螺内酯对大鼠肝纤维化?1(Ⅰ)、?1(Ⅲ)前胶原mRNA表达的影响
- Author:
Xin HUANG
;
Zheyong ZHANG
;
Dachun CAO
- Publication Type:Journal Article
- Keywords:
Spironolactone;
Hepatic fibrosis;
Procllagen
- From:
Chinese Journal of Practical Internal Medicine
2006;0(24):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of spironolactone on expression of ?1(Ⅰ) and ?1(Ⅲ) procollagen mRNA in rat hepatic liver fibrosis.Methods Fifty male SD rats were randomly divided into 3 groups.Hepatic fibrosis model group: the rats were injected with 400 mL/L CCl4 3 mL/kg subcutaneously two times a week for 10 weeks (Group A)or 13 weeks(Group B).Spironolactone group: the rats were injected with 400 mL/L CCl4 3 mL/kg subcutaneously two times a week.Spironolactone equivalent to 20 mg/kg per day was given intragastrically for 10 weeks(Group C)or 13 weeks(Group D).Normal control group: normal chow.The expression of ?1(Ⅰ) and ?1(Ⅲ) procollagen mRNA were detected by RT-PCR.Results At the end of week 10,the levels of ?1(Ⅰ)and ?1(Ⅲ) procollagen mRNA were significantly decreased in spironolactone group(P
