EFFECT OF PHYTIC ACID ON EXPRESSIONS OF APOPTOSIS ASSOCIATED PROTEIN FAS、BAX AND CASPASE-3 IN SGC-7901 CELLS
- VernacularTitle:植酸对SGC-7901细胞凋亡相关蛋白Fas、Bax及Caspase-3表达的影响
- Author:
Hongbin CUI
;
Zhiping YANG
;
Chuanying REN
- Publication Type:Journal Article
- Keywords:
phytic acid;
apoptosis;
Fas;
Bax;
caspase-3;
SGC-7901 cells
- From:
Acta Nutrimenta Sinica
2004;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of phytic acid on apoptosis of SGC-7901 cells and its mechanisms. Method:The inhibiting action of phytic acid on SGC-7901 cells was examed by MTT assay,and the effect on apoptosis of SGC-7901 cells was examined by DNA gel electrophoresis,and the expressions of Fas,Bax and caspase-3 protein were detected by Western blot method. Results:From MTT assay,we observed that phytic acid inhibited the growth of SGC-7901 cells in dose and time dependent manner. Typical DNA ladder was found during gel eletrophoresis. Caspase-3 can be activated by phytic acid and the expressions of Bax and Fas protein in phytic acid group were higher compared with control group,and increased in a dose-dependent manner. Conclusion:Phytic acid can induce apoptosis of SGC-7901 cells which may be correlated to apoptosis associated protein Fas,Bax and caspase-3.
