HUMAN TfR GENE EXPRESSION BEFORE AND AFTER IRON SUPPLEMENTATION IN WHITE BLOOD CELL DETECTED BY FLUORESCENCE QUANTITATIVE POLYMERASE CHAIN REACTION
- VernacularTitle:应用荧光定量PCR技术研究铁对白细胞TfR基因表达的调控
- Author:
Na LV
;
Ling ZHANG
;
Ning LIU
- Publication Type:Journal Article
- Keywords:
fluorescence quantitative PCR;
TfR;
white blood cell;
gene expression
- From:
Acta Nutrimenta Sinica
2004;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To quantitively detect TfR gene expression of human white blood cell before and after iron supplementation. Methods:Before and after iron supplementation,10 healthy women were phlebotomized separately,and the real-time fluorescence quantitative PCR method was used to analyze the expression difference of TfR gene in human white blood cell. Results:TfR gene expression decreased after iron supplementation in 10 women,more evidently in 7 cases,where the expression level was half less than before iron supplementation. Conclusion:TfR gene expression of human white blood cell could be quantitatively measured by FQ-PCR,and was decreased after iron supplementation in 10 women. It explained that the regulative mechanism of TfR mRNA expression by iron is also suitable to white blood cell.
