Effects of IFN-? on Expression of Smad3 mRNA in Liver Tissue of Rats with Hepatic Fibrosis
- VernacularTitle:?干扰素对实验性肝纤维化大鼠Smad3 mRNA的影响
- Author:
Xiaonan SUN
;
Guoqiang LOU
;
Xiankai WANG
- Publication Type:Journal Article
- Keywords:
IFN-?;
Smad3 protein;
Hepatic fibrosis;
Experimental;
Real-time RT-PCR;
Transforming growth factor?1
- From:
Journal of Medical Research
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Study the possible mechanisms of inhibiting hepatic fibrosis of IFN-? by observing the effects of IFN-? on the expression of smad3 mRNA in rat hepatic fibrosis model.Methods 70 rats were divided into three groups at random-fibrosis model group、IFN-? treatment group and normal control group.There are 30 rats in fibrosis model group which were induced to hepatic fibrosis by subcutaneous injection of carbon tetrachloride(CCl4) for 12 weeks.In the meantime,using IFN-? to treat the rats in IFN-? treatment group for 12 weeks.Histopathology changes and degree of fibrosis in livers of all rats were observed.Eventually,Smad3 mRNA were detected and quantified by real-time RT-PCR.Results In contrast with normal control group,the degrees of fibrosis in rat fibrosis model were significantly increased(P
