Function Analysis of Immunocytes in Synovial Fluid in Patients with Rheumatoid Arthritis
- VernacularTitle:类风湿关节炎患者关节滑膜液免疫细胞功能分析
- Author:
Keyi WANG
;
Hua ZHU
;
Guangchao ZHUO
- Publication Type:Journal Article
- Keywords:
Rheumatoid arthritis;
Costimulatory molecule;
Th1/Th2 cells;
Cytokines;
Lymphocyte activation markers
- From:
Journal of Medical Research
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the function of immunocytes in synovial fluid and the mechanisms of immune function disorder in patients with rheumatoid arthritis(RA).Methods The synovial fluid of 20 patients with RA and 10 healthy amputees because of traumatism in the affiliated hospital of Zhejiang university were collected in this syudy.Monoclonal antibodies against CD28,CD154(CD40L),CD80,CD86,CD40,CD69,CD25,HLA-DR,CD71 were used for flow cytometry and the expressions of costimulatory molecule CD28,CD154,CD80,CD86,CD40 on immunocytes and lymphocyte activation markers CD69,CD25,HLA-DR,CD71 in synovial fliud were studied in these patients.We also used enzyme linked immunosorbent assay(ELISA) to detect the cytokines level of Th1 cell including interleukine 2(IL-2),interferon-gamma(IFN-?) and those of Th2 cell including interleukine 6(IL-6),interleukine 10(IL-10) in the synovial fliud.Results Compared with control group,the expressions of CD80[(2.09?0.59)%vs(0.67?0.57)%,P
