Effects of carbachol on gastric emptying and gastric blood flow in oral fluid resuscitation of burn shock in rats
- VernacularTitle:卡巴胆碱对大鼠烫伤休克早期口服补液时胃排空和胃血流量的影响
- Author:
Sen HU
;
Bo HAO
;
Jinwei CHE
- Publication Type:Journal Article
- Keywords:
burns;
carbachol;
oral rehydration;
gastric emptying;
regional blood flow
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of carbachol(a cholinergic agent)on gastric emptying and gastric blood flow in resuscitation of early shock with oral administration of glucose-electrolyte solution(GES)in scalded rats.Methods Seventy-eight male Wistar rats were randomly assigned into five groups:shame scald(n=6),scald without fluid resuscitation(n=18),scald resuscitated with oral GES alone(n=18),scald resuscitated with oral carbachol alone(CAL,n=18),and scald resuscitated with oral carbachol plus GES(GES/CAL,n=18).About 35% TBSA scalded injury on back of rats was produced with boiling water(100℃,15s)in the latter four groups.GES(2ml/kg,which is equivalent to half volume of fluid infusion in first 24 hour according to Parkland' formula)was given twice in two-hour intervals by gavage beginning from 30min after the injury in GES and GES/CAL group.Carbachol(60?g/kg)was gavaged 30min after the injury in CAL and GES/CAL group.The gastric emptying rate was determined by phenol red emptying method and gastric blood flow was measured with laser Doppler at 2h,4h and 6h after the scald injury.Results The gastric emptying was obviously delayed and gastric blood flow was significantly reduced in scald animals compared with those in sham group(P
