Effect of leflunomide on NF-?B P65 in mouse to rat cardiac xenograft
- VernacularTitle:来氟米特在大鼠异种心脏移植排斥反应中对NF-?B P65的影响
- Author:
Guanglun YANG
;
Zhenxiang YAO
;
Ping HUANG
;
Gang TU
- Publication Type:Journal Article
- Keywords:
xenotransplantation;
heart transplantation;
immunosuppressant;
NF-?B
- From:Journal of Third Military Medical University
2003;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective By reproducing mouse to rat heart xenograft,to determine the effect of leflunomide on NF-?B P65 in the transplanted heart.Methods The recipient rats were randomly divided into 4 groups:group A(n=6)receiving no immunosuppressant as control;group B(n=6)cyclosporine(CsA);group C(n=6)leflunomide(Lef),group D(n=6)Lef plus CsA.The expressions of NF-?B P65 protein and NF-?B P65 DNA binding activity in transplanted heart tissues were determined by immunohistochemistry,Western blot and electrophoretic mobility shift assay(EMSA).Results Administration of CsA alone had no appreciable effect on the survival time of xenografts [(2.50?1.05)d] and could not suppress the protein expression of NF-?B P65 [(263.09?28.81),IOD] and NF-?B P65 DNA binding activity [(227.49?14.83),IOD] in the tissues of xenografts.Administration of Lef alone could significantly prolong the survival time of xenografts [(4.17?1.33)d] and could suppress the protein expression of NF-?B P65 [(173.48?5.85),IOD] and NF-?B P65 DNA binding activity [(109.96?12.46),IOD](P
