Methylation of DNA mismatch repair gene MLH1 and MSH2 in acquired multidrug-resistance of human small cell lung cells H446
- VernacularTitle:DNA错配修复基因甲基化在H446细胞获得性耐药中的作用
- Author:
Ruiling GUO
;
Guoming WU
;
Fuyun JI
;
Shungui XU
- Publication Type:Journal Article
- Keywords:
DNA mismatch repair;
MLH1;
MSH2;
SCLC;
acquired resistance;
promoter methylation
- From:Journal of Third Military Medical University
2003;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of methylated DNA mismatch repair gene MLH1 and MSH2 in the acquired multidrug-resistance of human small cell lung cancer cells H446.Methods The reverse transcription polymerase chain reaction(RT-PCR)and Western blot were applied to measure MLH1 and MSH2 mRNA and protein expressions of the multidrug-resistant cells H446/DDP and its parental cells H446.The promoter methylation status of the genes was assessed by methylation-specific PCR(MSP).Results The expressions of MLH1 and MSH2 significantly decreased both in mRNA level and protein level.Promoter methylation of MLH1 was observed in H446/DDP cells but not in H446 cells.Promoter semi-methylation of MSH2 in H446 cells was transformed to methylation in H446/DDP cells.Conclusion The downregulation of DNA mismatch repair gene MLH1 and MSH2 induced by its promoter methylation may play an important role in the acquired multidrug resistance of human small cell lung cancer.
