Differential microRNA expression profile of HepG2 cells induced by HBV integration
- VernacularTitle:HBV整合引起HepG2细胞microRNA表达谱变化分析
- Author:
Junfeng HAN
;
Zhihua RUAN
;
Bei ZHANG
;
Yuzhang WU
- Publication Type:Journal Article
- Keywords:
microRNA microarray;
HBV;
microRNA.
- From:Journal of Third Military Medical University
2003;0(22):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the differential expressions of microRNA after HBV integration. Methods A human microRNA microarray was used to analyze the microRNA expression profile in HepG2.2.15 cell line, a novel model of HBV infection. HepG2 cells served as a mock control. Then the microRNA with differential expression were proved using real-time PCR. Results HBV infection induced 6 microRNA down-regulated (hsa-miR-30a-5p, hsa-miR-24, hsa-let-7a, hsa-let-7c, hsa-let-7f and hsa-miR-23b) and 3 microRNA up-regulated (hsa-miR-194, hsa-miR-200a and hsa-miR-345). Conclusion HBV integration can induce the changes of microRNA expression profile in hepatocytes.
